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Figure 1 | BMC Plant Biology

Figure 1

From: Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Figure 1

Location of the T-DNA insertion in mutant line SALK_038124, and cassettes for expressing AtSUC2 from exotic promoters. A, T-DNA insertion site in the second intron of AtSUC2 in SALK_038124 with sequences at the junction between T-DNA and genomic DNA indicated. Nucleotide numbering is relative to the ATG start codon, and based on gene model AT1G22710.1 at http://www.arabidopsis.org. B, Schematic representation of cassettes for expressing AtSUC2 cDNA (cSUC2) as a native protein and as fusions with reporter enzymes; promoters, fusions, and restriction endonuclease recognition sequences as indicated; TGA, in frame stop codon flanked by restriction sites for creating the native AtSUC2 protein. The figure is not to scale.

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