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Table 2 Expression analyses of BnWRKY genes to five plant defense-related hormone treatments assayed by qRT-PCR.

From: Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments

gene

JA

ET

SA

ABA

BAP

 

6 h

24 h

6 h

24 h

6 h

24 h

6 h

24 h

6 h

24 h

BnWRKY1

2.47 (± 1.40)

1.11 (± 0.06)

0.83 (± 0.03)*

1.13 (± 0.03)*

1.53 (± 0.26)

1.10 (± 0.2)

2.54 (± 1.31)

1.03 (± 0.08)

0.59 (± 0.02)**

0.58 (± 0.08)*

BnWRKY6

0.64 (± 0.14)

0.88 (± 0.29)

1.07 (± 0.59)

3.98 (± 0.81)

1.99 (± 0.39)

1.29 (± 0.37)

2.17 (± 0.17)

1.23 (± 0.13)

0.72 (± 0.13)

0.64 (± 0.22)

BnWRKY11

0.63 (± 0.01)**

0.85 (± 0.12)

0.70 (± 0.00)**

1.02 (± 0.10)

1.63 (± 0.21)

1.22 (± 0.29)

1.16 (± 0.03)

1.06 (± 0.37)

0.64 (± 0.11)

0.97 (± 0.05)

BnWRKY18

1.99 (± 0.64)

1.31 (± 0.33)

1.74 (± 0.20)

1.60 (± 0.33)

11.65 (± 4.02)

6.48 (± 0.59)

3.01 (± 0.62)

1.07 (± 0.39)

0.71 (± 0.27)

0.38 (± 0.11)*

BnWRKY20

0.85 (± 0.24)

0.95 (± 0.12)

0.68 (± 0.04)*

1.38 (± 0.13)

1.38 (± 0.35)

1.19 (± 0.15)

0.77 (± 0.15)

0.87 (± 0.03)

0.61 (± 0.07)*

0.59 (± 0.14)

BnWRKY25

1.71 (± 0.54)

1.53 (± 0.16)

1.82 (± 0.16)

2.34 (± 0.50)

1.69 (± 0.39)

0.92 (± 0.09)

2.51 (± 1.45)

1.83 (± 0.19)*

0.55 (± 0.07)*

0.42 (± 0.13)*

BnWRKY28

0.75 (± 0.11)

1.55 (± 0.25)

0.86 (± 0.26)

1.49 (± 0.05)**

1.37 (± 0.45)

3.64 (± 3.00)

1.00 (± 0.09)

1.75 (± 1.02)

1.00 (± 0.38)

1.32 (± 0.73)

BnWRKY32

1.11 (± 0.15)

1.14 (± 0.15)

0.81 (± 0.04)*

1.33 (± 0.06)*

1.44 (± 0.34)

0.88 (± 0.08)

1.27 (± 0.17)

0.97 (± 0.12)

0.73 (± 0.04)*

0.82 (± 0.14)

BnWRKY33

0.68 (± 0.30)

0.76 (± 0.09)

3.89 (± 0.09)

2.38 (± 0.31)*

4.80 (± 1.23)

1.40 (± 0.36)

0.50 (± 0.15)

1.00 (± 0.21)

1.09 (± 0.17)

0.87 (± 0.18)

BnWRKY40

0.84 (± 0.2)

1.17 (± 0.44)

4.74 (± 0.05)*

6.49 (± 1.63)

2.17 (± 0.47)

0.99 (± 0.24)

1.28 (± 0.47)

1.69 (± 0.74)

0.61 (± 0.05)*

0.56 (± 0.16)

BnWRKY45

1.5 (± 0.51)

0.91 (± 0.21)

1.29 (± 0.17)

3.41 (± 0.39)*

1.39 (± 0.17)

1.11 (± 0.20)

2.35 (± 1.07)

1.71 (± 0.21)

0.63 (± 0.01)**

0.94 (± 0.21)

BnWRKY53

0.39 (± 0.14)

0.89 (± 0.50)

2.14 (± 0.07)

0.75 (± 0.10)

8.14 (± 1.69)

2.33 (± 1.05)

0.45 (± 0.00)**

0.81 (± 0.37)

1.43 (± 0.19)

2.08 (± 0.69)

BnWRKY65

1.41 (± 0.36)

1.58 (± 0.51)

1.82 (± 0.70)

1.46 (± 0.33)

1.64 (± 0.40)

1.84 (± 0.71)

0.77 (± 0.19)

1.16 (± 0.42)

0.78 (± 0.17)

0.50 (± 0.05)**

BnWRKY69

0.71 (± 0.10)

1.04 (± 0.27)

1.29 (± 0.01)**

1.76 (± 0.29)

1.29 (± 0.16)

1.15 (± 0.19)

0.42 (± 0.08)*

1.17 (± 0.41)

0.65 (± 0.08)*

0.56 (± 0.10)*

BnWRKY70

0.84 (± 0.16)

1.12 (± 0.21)

1.43 (± 0.24)

0.52 (± 0.00)**

13.98 (± 6.01)

3.66 (± 2.00)

0.85 (± 0.12)

1.01 (± 0.36)

0.46 (± 0.04)**

0.83 (± 0.26)

BnWRKY75

1.55 (± 0.36)

2.30 (± 0.93)

2.39 (± 0.03)*

9.21 (± 0.63)*

17.58 (± 12.54)

8.19 (± 4.61)

4.53 (± 2.14)

2.03 (± 0.43)

0.50 (± 0.24)

0.60 (± 0.30)

  1. Results are presented as a ratio of transcript abundance in treatment/mock on a linear scale. Data were mean of three biological replicates ± S.E. The asterisk indicates that the corresponding gene was significantly up- or down-regulated under a stress treatment by t-test (* for p < 0.05 and ** for p < 0.01).