Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)

Dracatos, Peter M; Cogan, Noel OI; Sawbridge, Timothy I; Gendall, Anthony R; Smith, Kevin F; Spangenberg, German C; Forster, John W

doi:10.1186/1471-2229-9-62

Table 1 Classification of primary R gene templates used for host-specific genetic analysis, according to isolation strategy

From: Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)

Perennial ryegrass unique identifier (UI)	Source of primary R gene template sequence	Reference
Primer design based on Pooideae R gene templates
LpLrk10	Wheat leaf rust receptor kinase	[41]
LpPcaClone1	Oat NBS-LRR candidate from Pca cluster	[37]
LpPcaClone2	Oat NBS-LRR candidate from Pca cluster
LpPcaClone3	Oat NBS-LRR candidate from Pca cluster
LpPcaClone4	Oat NBS-LRR candidate from Pca cluster
LpHvClone1	Barley NBS-LRR co-locating with QTL	[33]
LpHvClone2	Barley NBS-LRR co-locating with QTL
LpHvClone3	Barley NBS-LRR co-locating with QTL
LpHvClone4	Barley NBS-LRR co-locating with QTL
LpHvClone5	Barley NBS-LRR co-locating with QTL
*Degenerate primer pair design*
LpRGcontig1	Degenerate primer pair pair	Additional File 1
LpRGcontig2	Degenerate primer pair
LpRGcontig3	Degenerate primer pair
LpRG1NBS	Degenerate primer pair
LpRG2NBS	Degenerate primer pair
LpRG3NBS	Degenerate primer pair
LpRG4NBS	Degenerate primer pair
LpRG5NBS	Degenerate primer pair
LpRG6NBS	Degenerate primer pair
LpRG7NBS	Degenerate primer pair
LpNBS-LRR1	Degenerate primer pair
LpNBS-LRR2	Degenerate primer pair
LpNBS-LRR3	Degenerate primer pair
LpNBS-LRR4	Degenerate primer pair
LpNBS-LRR5	Degenerate primer pair
LpNBS-LRR6	Degenerate primer pair
LpNBS-LRR7	Degenerate primer pair
LpNBS-LRR8	Degenerate primer pair
LpNBS-LRR9	Degenerate primer pair
LpNBSC1	Degenerate primer pair
LpNBSC2	Degenerate primer pair
LpNBSC5	Degenerate primer pair
LpNBSC8	Degenerate primer pair
LpNBSC15	Degenerate primer pair
LpDEGVed1_d03_gp08	Degenerate primer pairs designed to oat NBS
LpDEGVed2_d07_gp09	Degenerate primer pairs designed to oat NBS
LpDEGVed3_a11_gp09	Degenerate primer pairs designed to oat NBS
LpDEGVed4_d02_gp08	Degenerate primer pairs designed to oat NBS
*Primer design for candidate* Lolium *R genes*
LpESTa03_10rg	LpEST from bioinformatic discovery	[46]
LpESTa08_14rg	LpEST from bioinformatic discovery
LpESTa10_13rg	LpEST from bioinformatic discovery
LpESTb02_05rg	LpEST from bioinformatic discovery
LpESTb06_11rg	LpEST from bioinformatic discovery
LpESTc10_19rg	LpEST from bioinformatic discovery
LpESTd08_13rg	LpEST from bioinformatic discovery
LpESTe01_10rg	LpEST from bioinformatic discovery
LpESTe11_14rg	LpEST from bioinformatic discovery
LpESTf06_19rg	LpEST from bioinformatic discovery
LpESTf11_11rg	LpEST from bioinformatic discovery
LpESTg01_20rg	LpEST from bioinformatic discovery
LpESTg04_17rg	LpEST from bioinformatic discovery
LpESTg06_13rg	LpEST from bioinformatic discovery
LpESTh04_17rg	LpEST from bioinformatic discovery
LpESTh05_28rg	LpEST from bioinformatic discovery
LpESTh07_17rg	LpEST from bioinformatic discovery
LPCL_38150	LpEST from bioinformatic discovery
LPCL_8913	LpEST from bioinformatic discovery
LpHvESTClone1	LpEST from bioinformatic discovery
LpHvESTClone2	LpEST from bioinformatic discovery
LpHvESTClone3	LpEST from bioinformatic discovery
LpHvESTClone4	LpEST from bioinformatic discovery
LpAG205017	RG sequence from Italian ryegrass	[39]
LpAG205018	RG sequence from Italian ryegrass
LpAG205035	RG sequence from Italian ryegrass
LpAG205050	RG sequence from Italian ryegrass
LpAG205055	RG sequence from Italian ryegrass
LpAG205063	RG sequence from Italian ryegrass

LpEST = perennial ryegrass EST; RG = resistance gene.

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ISSN: 1471-2229

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