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Figure 7 | BMC Plant Biology

Figure 7

From: In planta localisation patterns of MADS domain proteins during floral development in Arabidopsis thaliana

Figure 7

Localisation of SEP3:GFP, AG:GFP and expression of pWUS:GUS during ovule development. Confocal microscopic analysis of SEP3:GFP localisation (A, D, and G) and AG:GFP localisation (B, E, and H) during ovule development. GFP tagged MADS domain proteins are detected as green signal and cell membranes are stained with the red dye FM4-64. DIC microscopy of pWUS:GUS expression in developing ovules (C, F, and I) where GUS activity is detected as a blue colour. (A) SEP3:GFP signal in the nucellus, chalaza, and funiculus of an ovule at stage 2-II. (B) AG:GFP signal in the chalaza and funiculus of an ovule at stage 2-II. (C) pWUS:GUS expression in an ovule at stage 2-II in an increasing basal-to-apical gradient. (D) High SEP3:GFP signal specifically in the nucellus, the inner integument, and the funiculus in an ovule at stage 2-III. (E) AG:GFP signal in an ovule at stage 2-III, with beginning signal in the nucellus and the highest signal in the developing inner and outer integuments. (F) pWUS:GUS expression in the nucellus and the two integuments of an ovule at stage 2-III in an increasing basal-to-apical gradient. (G) SEP3:GFP signal in an ovule at stage 2-IV with the highest signal in the nucellus. (H) AG:GFP signal in the nucellus, the two integuments, and the funiculus of an ovule at stage 2-IV. (I) pWUS:GUS expression only in the nucellus in an ovule at stage 2-IV. (c) chalaza; (f) funiculus; (ii) inner integument; (oi) outer integument; (n) nucellus. All scale bars 10 μm.

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