Confocal microscopic analysis of FUL:GFP localisation in inflorescence meristem and early flower bud stages. (A) Overview of an inflorescence with the inflorescence meristem and early flower bud stages 2 to 7 indicated. FUL:GFP protein is detected as green signal and cell membranes are stained with the red dye FM4-64. (B) Section through an inflorescence meristem showing high FUL:GFP signal in all layers, while stage 1 and 2 flower bud primordia have reduced signal. (C) Detail of a stage 3 flower bud showing the highest FUL:GFP signal in the floral meristem and only epidermal signal in the four sepals. (D) Detail of a stage 5 flower bud showing the highest FUL:GFP signal in whorl 4 and reducing signal in the petal and stamen primordia. (2–7) flower bud stages; (FM) floral meristem; (IM) inflorescence meristem; (p) petal; (s) sepal; (st) stamen; (w4) whorl 4. Scale bar of (A) 50 μm and scale bars of (B-D) 25 μm.