Confocal microscopic analysis of AG:GFP localisation in inflorescence meristem and early flower bud stages. (A) Overview of an inflorescence with the inflorescence meristem and early flower bud stages 2 to 6 indicated. AG:GFP protein is detected as green signal and cell membranes are stained with the red dye FM4-64. (B) Detail of a stage 4 flower bud showing AG:GFP signal in the future third and fourth whorl. (C) Section through the stage 4 flower bud in (B) showing that AG:GFP is located in both the cytoplasm and the nuclei. (D) Detail of the stage 5 flower bud in (A) showing AG:GFP signal in the developing stamen and in whorl 4. (2–6) flower bud stages; (FM) floral meristem; (IM) inflorescence meristem; (p) petal; (s) sepal; (st) stamen; (w4) whorl 4. Scale bar of (A) 50 μm and scale bars of (B-D) 25 μm.