Transcriptional expression of selected fungal genes during the infection process. Expression ratio of selected U. appendiculatus genes during the first 96 hours of the infection with bean rust race 49 or 41. qRT-PCR was performed on three independent biological replicates using Cons7 as a reference for normalization. Six ESTs, CL2800Contig1 (heat shock protein 90), CL1917Contig1 (proteasome subunit alpha), CL2317Contig1 (Glutamine synthetase), CL1Contig289 (Asparaginyl-tRNA synthetase), CL3018Contig1 (planta-induced rust protein 1), and CL4935Contig1 (unknown), were found strongly up-regulated in tissues infected with the fungal race 41 in comparison to tissues infected with the fungal race 49. The tag identification for these ESTs is "late race 41" indicating that they came originally from tissue infected with race 41. *: data significant with 0.05 < p-value ≤ 0.1. **: data significant with 0.01 < p-value ≤ 0.05. ***: data significant with p-value ≤ 0.01. nd: not determined.