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Table 1 Frequencies of BY-2 calli and suspensions with homogeneous and heterogeneous GFP fluorescence

From: Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression

   Primary suspensions   Secondary suspensions
GFP fluorescence in callus Primary calli Homogeneous Heterogeneous Secondary calli Homogeneous Heterogeneous
Homogeneous 39.3% ± 9.7% 29.2% ± 5.3% 70.8% ± 5.3% 93% ± 2.3% 46.3% ± 5.4% 53.7% ± 5.4%
Heterogeneous – mosaic 35.8% ± 13% 0% 100% 7% ± 2.3% 0% 100%
Heterogeneous – sectorial 24.9% ± 6.8% 0% 100% 0% 0% 100%
   11.5% in total 88.5% in total   42.8% in total 57.2% in total
  1. Frequencies of homogeneous and heterogeneous calli and suspension cultures derived from these calli in primary lines obtained after transformation, and in secondary lines produced by cloning of primary heterogeneous (!) suspensions. Values represent means ± SD (n = 3); data are from three independent transformations; in every replication the number of evaluated lines was 60–80 for calli and ~20 for suspensions).