Figure 3

Distribution of TRRT in the tomato genome. (A) Chromosomal localization of TRRT (red signal). FISH analysis of pachytene stage S. lycopersicum was probed with TRRT. TRRT was localized on the heterochromatin of all chromosome include NOR of chromosome 2 (arrow). Bar, 10 μm (B) Fiber FISH shows that the TRRTs (red signal) are localized in the rDNA array (pTA71, green signal). (C) Arrows indicate the direction of seven TRRTs present in the HBa007F24 clone. TRRT in unit IV has solo LTR sequence. Blue box (18S rDNA) and green box (25S rDNA) indicate fragmented rRNA genes. (D) Comparison of flanking sequences of TRRTs with those of typical 18S rRNA genes. Seven TRRTs are inserted in the same site and create 4 bp TDS (shadow boxes). (E) Organization of TRRT.