Expression and localization of ptCpn60β and FtsZ in plastid cpn60 mutants. (A) Immunoblot analyses using anti-ptCpn60β and anti-FtsZ2-1 antibodies. Total proteins extracted from seedlings of wild type, ptcpn60β1-1, ptcpn60β2, ptcpn60β1-1 ptcpn60β2, and ftsZ2-1 in mesophyll cells were blotted. (B) RT-PCR analyses comparing transcript levels of ptCpn60β1 and ptCpn60β2. cDNA was prepared from total RNA extracted from the wild type, ptcpn60β1-1, and ptcpn60β2. (C) Localization of ptCpn60β in wild type and of FtsZ2-1 in wild type, ptcpn60β1-1, and ptcpn60α1-1 in mesophyll cells was examined by immunofluorescence microscopy. Scale bars = 10 μm.