Comparison of phenotypes between two ptcpn60α1 mutants and in combinations with ptcpn60β1-1 and ptcpn60β2 mutants. (A-E) Seedlings, chloroplasts in leaf mesophyll cells, and chlorophyll contents of ptcpn60a1 mutants. Phenotypes of ptcpn60α1–2 (SALK_006606) were complemented by a ptCpn60α transgene (D). (F-H) The seedlings, chloroplasts in leaf mesophyll cells, and chlorophyll contents in plants with combinations of ptcpn60β1-1 and ptcpn60β2 mutations. +/+, wild type. +/-, heterozygous mutant. -/-, homozygous mutant. Scale bars = 2 mm (A-D, left panels), 10 μm (A-D, right panels), 2 mm (F), and 10 μm (G). Error bars represent the standard deviation (E, H). n.d., not determined (H).