Figure 4From: Cbfgenes of the Fr-A2 allele are differentially regulated between long-term cold acclimated crown tissue of freeze-resistant and – susceptible, winter wheat mutant linesConfirmation of microarray expression pattern by RT-PCR. Total RNA isolated from crown tissue of FR and FS winter wheat lines and primers (Table 2) were used to determine Cbf-3, -5 and -12 expression levels. Values were calculated with the MXPro-Mx300P Stratagene software with the 18S levels as normalizers and the FR/NT levels as the calibrator. The relative quantity of gene expression for NT = untreated or non-treated samples, CA = cold-acclimated samples of FR and FS lines are depicted.Back to article page