Table 3 Primer information table.
From: Plant origin and ploidy influence gene expression and life cycle characteristics in an invasive weed
Name (Unigene ID) | Primer sequence (5'-3') | homologs | references |
|---|---|---|---|
Secondary Metabolism | |||
PAL 1 phenylalanine ammonia lyase (03772, 01487, 04157, 00435, 00996) | GAAATGGACCCGTTGCAGAAGCC GCTTCGGCTGTTTTTCTTGCGGAAAT | PAL 1 Arabidopsis At2g37040 Lactuca AAL55242 | Olsen et al. 2008 [56] Rookes et al. 2003 [57] Winkel- |
PAL 2a (00151) | AGCTCCACCCCTCGAGATTC GTCACCTTCTCACCGGTCAA | PAL 2 Arabidopsis At3g53260 Lactuca AAO13347 | Shirley 2001 [34] |
PAL 2b (04127) | ATCGCGAGTACTTCTTCGCC GTCACCTTCTCACCGGTCAA | PAL 2 Arabidopsis At3g53260 Lactuca AAO13347 | La Camera et al. 2004 [32] |
Defense-related | |||
Chitinase (00271, 03889, 03038, 04202, 03133) | TGGCTCCATCGTTACTGCATCTG AGTTGTGGGATAGCTGGATAGGTC | Chitinase Helianthus AAB57694 Chitinase, class II Arabidopsis At4g01700 | Kasprzewska 2003 [37] Jwa et al. 2006 [36] |
Glucanase (01113, 00896, 00032) | CGACCCGGTTAACATCAAGCTCG CGTCGAAAACTCCGTCGTCTTACC | Beta-1, 3-glucanase Arabidopsis At4g14080 | Doxey et al. 2007 [35] |
Standards | |||
Actin (01058) | ACCAACATGAGAACAACCGATAC TCACACTGGTGTCATGGTCGGAAT | Actin Gossypium hirsutum AAP73454 | |
Cytochrome C oxidase (Weller et al. 2000) | CGTCGCATTCCAGATTATCCA CAACTACGGATATATAAGAGCCAAAACTG | Weller et al. 2000 [60] | |
Ubiqutin | ACAACATCCAGAAGGAGTCC GCAACACAGCAAGCTTAACC | ||