Table 2 Test primer pairs used for semi quantitative RT-PCR amplification: names, TAIR database numbers, and predicted product sizes in bp are listed.
name | function | TAIR ID | sense primer | antisense primer | bp |
|---|---|---|---|---|---|
PATP | proteasome subunit | AT5G19990 | GGCGTCCTGAGACAGCGATGGAG | CAGGCCTGAGAAGAGCTTGATCCAG | 938 |
GS | cytosolic glutamine synthetase | AT1G66200 | GAAGGATGTGAACTGGCCTCTTG | GTAAGGGTCCATGTTTGAAGCTG | 615 |
PDIOX | pathogen inducible dioxygenase | AT3G01420 | GTATGCGACGCCCTCAAGGATG | CCTTGAGACTCTCTGTAGTATTCACC | 936 |
MTH | homocysteine methyltransferase | AT5G17920 | GCTGATCTCAGGTCATCCATCTG | GATTGAGCTTCTTCTGCTGAGCATC | 1170 |
MDH | malate dehydrogenase | AT5G09660 | GGAAAACTGCAGAGCTAAAGGTGG | CCAAGCTGATACACTTCCTCTGC | 878 |
PRX42 | peroxidase 42 | AT4G21960 | GACCACAACGAGAGTATCTCCGTC | CAAGCAGAGAACTCAACACACCAGAG | 544 |
RD21A | cysteine proteinase (RD21A) | AT1G47128 | GTGAGAGAAGGACTAGCCTACGGTAC | CACAAACCGGGTACTCGTGAG | 914 |
3OA | 3-oxoacyl-(acyl-carrier protein) | AT1G24360 | GATGAAAACCGCTCTTGACAAATG | CATCAATGGTGAATGCCTGTCC | 511 |
MO1 | aromatic-ring hydroxylase | AT4G15760 | GTTTGCTTGTCGTGCGGTGAGAG | CTGCACCGAAAGCCCGAGTAATC | 555 |
PR1 | PR1 pathogen related | AT2G14610 | TCGTCTTTGTAGCTCTTGTAGGTG | TAGATTCTCGTAATCTCAGCTCT | 590 |
GLIP1 | lipase, defense response | AT5G40990 | CGATTGTGCACCAGCCTCATTGGTT | CAGCGCTTTGAGATTATAGGGTCC | 429 |
PR2 | PR2 pathogen-related, cellulase | AT3G57260 | CGTTGTGGCTCTTTACAAACAACAAAAC | GAAATTAACTTCATACTTAGACTGTCGAT | 870 |
PR3 | PR3 defense response chitinase | AT3G12500 | CGGTGGTACTCCTCCTGGACCCACCGGC | CGGCGGCACGGTCGGCGTCTGAAGGCTG | 583 |
PR4 | PR4 hevein-like defense protein | AT3G04720 | GACAACAATGCGGTCGTCAAGG | AGCATGTTTCTGGAATCAGGCTGCC | 552 |
PR5 | PR5 thaumatin-like | AT1G75040 | ATGGCAAATATCTCCAGTATTCACA | ATGTCGGGGCAAGCCGCGTTGAGG | 484 |
PDF1.2 | defensin | AT5G44420 | GCTAAGTTTGCTTCCATCATCACCCTT | AACATGGGACGTAACAGATACACTTGT | 237 |
EIN2 | ethylene signal transduction | AT5G03280 | TGCAGCTCGCATAAGCGTTGTGACTGGTA | CGCTCTCTCCATTTAACCGAGTTAACAC | 379 |
EIN3 | ethylene signal transduction | AT3G20770 | GATGTTGATGAATTGGAGAGGAGGATG | ACGTCTCTGAGGAGGATCACAGTGT | 470 |
ERF1 | ethylene response factor | AT3G23240 | CGGCTTCTCACCGGAATATTCTATCG | TCTCCGAAAGCGACTCTTGAACTCTCT | 415 |
ACT2 | actin 2 | AT3G18780 | TCACCACAACAGCAGAGCGGG | GGACCTGCCTCATCATACTCGG | 257 |
UBC | ubiquitin conjugating | AT5G25760 | GGCATCAAGAGCGCGACTGTT | CTTTCTTAGGCATAGCGGCGAG | 217 |
CBP20 | Cap-binding protein 20 | AT5G44200 | TTGTGGCTTTTGTTTCGTCCTG | CGTGGGTTCTTCTCCGGTCTC | 409 |