Figure 8

Changes in the ratio of nuclear to chloroplast DNA and chloroplast genomes (plastomes) per cell using qPCR. (A) Mean ploidy level (C-value) determined by flow cytometry (B) Ratio of the amount of copies of the chloroplast psbA gene relative to that of the multiple copy nuclear 18S rRNA gene calculated using the 2^-ΔCT method of qPCR analysis. (C) Ratio of the amount of copies of the chloroplast psbA gene relative to that of the single copy nuclear ROC1 gene calculated using the 2^-ΔCT method of qPCR analysis. (D) Plastome copy number per cell assessed by multiplying the ratio of psbA copies to 18S rRNA copies (described in (B)) by the mean number of 18S rRNA genes per haploid nuclear genome (see text) and by the mean ploidy level of the tissues (see Table 1). (E) Plastome copy number per cell assessed by multiplying the ratio of psbA copies to ROC1 copies (described in (C)) by the mean ploidy level of the tissues (see Table 1). Tissue samples 1–6 are described in the legend of Table 1.