Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets

Table 5 Expression of genes encoding enzymes involved in ascorbate-glutathione cycles after 24 hours of treatment.

			Log₂(ratio)
Accession number	Gene description	Localisation	Treatment Comparison
			S/M	MA/M	SA/M
At1g07890	L-ascorbate peroxidase 1. cytosolic (APX1)	Cytosol	nde	-1.92	nde
At1g77490	L-ascorbate peroxidase. thylakoid-bound (tAPX)	Chloroplast	-1.03	nde	-1.08
At3g09640	L-ascorbate peroxidase 2 (APX2)	Cytoplasm	nde	nde	nde
At4g08390	L-ascorbate peroxidase. stromal (sAPX)	Chloroplast	1.46	-1.18	nde
At4g09010	L-ascorbate peroxidase 4 (APX4)	Chloroplast	-0.79	-1.12	-1.40
At4g35000	L-ascorbate peroxidase 3 (APX3)	Peroxisome	nde	nde	nde
At1g75270	Dehydroascorbate reductase (DHAR2)	Cytoplasm	1.92	-0.91	2.70
At5g16710	Dehydroascorbate reductase (DHAR3)	Chloroplast	nde	nde	nde
At5g36270	Dehydroascorbate reductase. putative	Cytoplasm	0.80	nde	1.20
At1g63940	Monodehydroascorbate reductase (MDAR5)	Chloroplast	nde	nde	nde
At3g09940	Monodehydroascorbate reductase (MDAR3)	Cytoplasm	nde	nde	nde
At3g27820	Monodehydroascorbate reductase (MDAR4)	Cytoplasm	nde	nde	nde
At3g52880	Monodehydroascorbate reductase (MDAR1)	Cytoplasm	nde	nde	nde
At5g03630	Monodehydroascorbate reductase (MDAR2)	Cytoplasm	nde	-1.35	1.11
At3g24170	Glutathione reductase. putative (GR1)	Cytoplasm	1.15	nde	0.92
At3g54660	Gluthatione reductase. chloroplast (GR2)	Chloroplast	nde	nde	nde

Relative expressions of gene are given with their log₂(ratio) for sucrose versus mannitol (S/M), mannitol plus atrazine versus mannitol (MA/M) and sucrose plus atrazine versus mannitol (SA/M) comparison. nde: not differentially expressed. Genes with a Bonferroni P-value higher than 5% were considered as being not differentially expressed as described by Lurin et al. [85].

ISSN: 1471-2229