Figure 2

Activities of metabolic enzymes in alamethicin-permeabilized Col-0 cells. Rates are expressed as percent of the highest rate in each experiment. (A) Effect of alamethicin on activities of PEPC, GAPDH, and NAD-IDH. The average maximum activity was for PEPC 480 ± 220 nmol min^-1 g^-1(FW), for GAPDH 1650 ± 300 nmol min^-1 g^-1(FW), and for NAD-IDH 210 ± 140 nmol min^-1 g^-1(FW). Averages of two independent experiments with error bars representing S.D. are shown. (B) Effect of alamethicin on activities of cytosolic and plastidic G6PDH. Averages are shown for three independent experiments with error bars representing S.E. The average maximum activity was 260 ± 50 and 420 ± 160 nmol min^-1 g^-1(FW) for cytosolic and plastidic G6PDH, respectively.