| |
WT
40-dCT |
exo
40-dCT | ddCT |
FC
|
|---|
10 d | | | | |
EXP5
| 38.4 ± 0.02 | 37.0 ± 0.02 | 1.4 | 2.6 |
KCS1
| 39.0 ± 0.02 | 37.9 ± 0.01 | 1.1 | 2.1 |
30 d
| | | | |
EXP5
| 37.2 ± 0.19 | 36.2 ± 0.01 | 1.1 | 2.1 |
KCS1
| 37.7 ± 0.43 | 36.4 ± 0.01 | 1.3 | 2.5 |
50 d
| | | | |
EXP5
| 35.1 ± 0.12 | 34.4 ± 0.17 | 0.7 | 1.6 |
KCS1
| 32.0 ± 0.12 | 31.5 ± 0.06 | 0.6 | 1.5 |
- Wild-type and exo plants were grown in soil. RNA was extracted from rosette leaves of 10-, 30-, and 50 day-old plants. eIF1α CT values were subtracted from respective CT values of the gene of interest resulting in dCT. Subsequently, differences were subtracted from an arbitrary value (i.e., 40). Higher numbers indicate higher transcript levels. ddCT results from subtracting (40 – dCT)
exo
from (40 – dCT)WT. A ddCT of one cycle indicates a fold change (FC, wild-type vs. exo) of two. Error: SE of gene of interest in three technical replicates. The experiment was carried out independently three times, representative examples of observed values are shown. For sequences of real-time RT-PCR primer pairs see [7].
