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Table 2 Comparative analysis of the 5' flanking and coding sequence characteristics in Glu-A1-2 alleles from diploid, tetraploid and hexaploid wheats.

From: Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions

    

Sequence characteristics

 

HWM-GS alleles

Species

Genome

Gene expression

5'flanking regions

Coding Region

References

1Ay (Tu-e1)

T. urartu

AA

active

85 bp deletion

 

This study

1Ay (Tu-e2)

T. urartu

AA

active

85 bp deletion

 

This study

1Ay (Tu-s)

T. urartu

AA

inactive

85 bp deletion

Stop codon

This study

1Ay (Ta-e1)

T. monococcum aegilopoides

AA

active

85 bp deletion

 

This study

1Ay (Ta-e2)

T. monococcum aegilopoides

AA

active

85 bp deletion

 

This study

1Ay (Ta-e3)

T. monococcum aegilopoides

AA

active

85 bp deletion

 

This study

1Ay (Ta-e)

T. monococcum aegilopoides

AA

inactive

85 bp deletion

Stop codon

This study

1Ay (Td-e)

T. turgidum dicoccon

AABB

active

85 bp deletion

 

This study

1Ay (Td-s)

T. turgidum dicoccon

AABB

inactive

85 bp deletion

Stop codon

This study

1Ay (Cheyenne)

T. aestivum

AABBDD

inactive

85 bp deletion

Stop codon

Forde et al. (1985)

1Ay (Chinese spring)

T. aestivum

AABBDD

inactive

85 bp deletion

transposon-like insertion

Harberd et al. (1987)

  1. Both active and inactive 1Ay genes from T. urartu, T. monococcum aegilopoides, T. turgidum dicoccon and T. aestivum shared high homology promoter sequences including 85 bp deletions. Nevertheless, the defects (premature stop codons and transposon-like insertion) were found in the coding regions of all silenced 1Ay genes.