Figure 4

Effects of TMV-Cg and ORMV infections on the accumulation of miR164 and CUC1 and CUC2 mRNAs. (A) Northern blot analysis detecting the accumulation of miR164 in transgenic lines L35 and L56 after virus infection. Ethidium-bromide-stained rRNAs shown below each blot were used for data normalization. miR accumulation data were set relative according to the accumulation in mock-inoculated plants that was set at 1. (B) Average of two to four independent measurements of miR164 accumulation in L56 and L35 after virus infection. (C) CUC1 and CUC2 mRNAs transcript abundance determined by qRT-PCR and expressed in arbitrary units normalized to EF1α amount after virus infection. CUC1 and CUC2 transcript levels were computed relative to the levels in mock-inoculated plants that were set at 1. Each value represents the mean of four biological replicates. Bars indicate standard errors. (*) indicates a statistically significant difference (p < 0.008) for CUC1 relative expression in TMV-Cg and ORMV-infected plants compared to controls ones.