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Table 4 Retrotransposon specific primers used in RT-PCR reactions.

From: Copia and Gypsy retrotransposons activity in sunflower (Helianthus annuus L.)

RT-PCR primers (Copia and Gypsy)
G13F 5'-TCAGACGGATGGGCAGTCTGAGCGA-3'
G13R 5'-ACTCTGGGCCACGACGGGAGTTCCA-3'
G22F 5'-ACGCGTTTGAACTTCAGTACGGCT-3'
G22R 5'-TCAGGACCTCTACGAGCATCC-3'
G30F 5'-GGTCTATCACCGGGTCTCAAC-3'
G30R 5'-TACCCGGAAATAATCGAAGTCGTG-3'
C211F 5'-GCTGGATGTCAGTTCTTAGG-3'
C211R 5'-GATTTCGATGTGTTTGGTCTT-3'
LTR isolation primers (Copia, Gypsy, and universal)
Copia ChWP3 5'-CGAGATGAGTGCGATGGGTGAAAT-3'
Gypsy ChWP4 5'-GCAGAGGTGGGAGATAGTCAGAT-3'
Anch. p 5'-ACCATCGTCCTCAGGTTAGTCAGG-3'
PBSmet 5'-TAGGTCGGAACAGGCTCTGATACCA-3'
P1FCo 5'-CTGGTGTCTGTAACTTGTCTGTATTCG-3'
P2FCo 5'-AAAGATATGCTTCGATTGATAGACCTC3'
P1FGy 5'-GTGAGTACGTACCAAATTTCGGGAC-3'
P2FGy 5'-TTTCAACTTGGGGATAATGTGACAAC -3'
IRAP primers (Copia)
FA C-LTR i-r 5'-AGAGCATTCTGTCCGAAACAC-3'
FC C-LTR-h 5'-TAGCTTGGATTCCGCACTCG-3'
FF C-LTR 5'-GGTTTAGGTTCGTAATCCTCCGCG-3'
C-LTR r-rt1 5'-CGATAGATGGTCCGAAGGATC-3'
C-LTR 1 5'-AGACACCAGTGGCACCAACA-3'
C-LTR 2 5'-ACAGACACCAGTGGCACCAAC-3'
IRAP primers (Gypsy)
1F G-LTR BO37 5'-GGACAATATCATGGTGCGGTTAC-3'
1F G-LTR DO48 5'-ACCCTTCTTGACGAGACCAGT-3'
G-LTR 1 5'-CTGGTTTTCCTGGGGTGTCA-3'
G-LTR 2 5'-GGGTTGTCACATTATCCCCAAG-3'
Polymorphic band analysis primers (Copia)
FF C-LTR 5'-GGTTTAGGTTCGTAATCCTCCGCG-3'
C-LTR 2 5'-ACAGACACCAGTGGCACCAAC-3'
EMB 1F 5'-TCTTGACATGGGTTGTGGGCT-3'
EMB 1R 5'-TCACATGAACACGGCTCACACA-3'
EMB 2F 5'-AGTCTAATGGGTCAGCATGG-3'
EMB 2R 5'-TCCCTGGTATGAGCCGAAGCTCT-3'