From: Copia and Gypsy retrotransposons activity in sunflower (Helianthus annuus L.)
RT-PCR primers (Copia and Gypsy) | |
---|---|
G13F | 5'-TCAGACGGATGGGCAGTCTGAGCGA-3' |
G13R | 5'-ACTCTGGGCCACGACGGGAGTTCCA-3' |
G22F | 5'-ACGCGTTTGAACTTCAGTACGGCT-3' |
G22R | 5'-TCAGGACCTCTACGAGCATCC-3' |
G30F | 5'-GGTCTATCACCGGGTCTCAAC-3' |
G30R | 5'-TACCCGGAAATAATCGAAGTCGTG-3' |
C211F | 5'-GCTGGATGTCAGTTCTTAGG-3' |
C211R | 5'-GATTTCGATGTGTTTGGTCTT-3' |
LTR isolation primers (Copia, Gypsy, and universal) | |
Copia ChWP3 | 5'-CGAGATGAGTGCGATGGGTGAAAT-3' |
Gypsy ChWP4 | 5'-GCAGAGGTGGGAGATAGTCAGAT-3' |
Anch. p | 5'-ACCATCGTCCTCAGGTTAGTCAGG-3' |
PBSmet | 5'-TAGGTCGGAACAGGCTCTGATACCA-3' |
P1FCo | 5'-CTGGTGTCTGTAACTTGTCTGTATTCG-3' |
P2FCo | 5'-AAAGATATGCTTCGATTGATAGACCTC3' |
P1FGy | 5'-GTGAGTACGTACCAAATTTCGGGAC-3' |
P2FGy | 5'-TTTCAACTTGGGGATAATGTGACAAC -3' |
IRAP primers (Copia) | |
FA C-LTR i-r | 5'-AGAGCATTCTGTCCGAAACAC-3' |
FC C-LTR-h | 5'-TAGCTTGGATTCCGCACTCG-3' |
FF C-LTR | 5'-GGTTTAGGTTCGTAATCCTCCGCG-3' |
C-LTR r-rt1 | 5'-CGATAGATGGTCCGAAGGATC-3' |
C-LTR 1 | 5'-AGACACCAGTGGCACCAACA-3' |
C-LTR 2 | 5'-ACAGACACCAGTGGCACCAAC-3' |
IRAP primers (Gypsy) | |
1F G-LTR BO37 | 5'-GGACAATATCATGGTGCGGTTAC-3' |
1F G-LTR DO48 | 5'-ACCCTTCTTGACGAGACCAGT-3' |
G-LTR 1 | 5'-CTGGTTTTCCTGGGGTGTCA-3' |
G-LTR 2 | 5'-GGGTTGTCACATTATCCCCAAG-3' |
Polymorphic band analysis primers (Copia) | |
FF C-LTR | 5'-GGTTTAGGTTCGTAATCCTCCGCG-3' |
C-LTR 2 | 5'-ACAGACACCAGTGGCACCAAC-3' |
EMB 1F | 5'-TCTTGACATGGGTTGTGGGCT-3' |
EMB 1R | 5'-TCACATGAACACGGCTCACACA-3' |
EMB 2F | 5'-AGTCTAATGGGTCAGCATGG-3' |
EMB 2R | 5'-TCCCTGGTATGAGCCGAAGCTCT-3' |