Table 2 Mutations found in 14 gene fragments analyzed in TILLer collection.
Gene | Amplicon length (bp) | Exon length (bp) | Intron length (bp) | GC content (%) | # of screened lines | Total # of mutations | Mutation class* | ||
|---|---|---|---|---|---|---|---|---|---|
Silent | Missense | Truncation** | |||||||
At1g12650 | 977 | 416 | 561 | 35.6 | 1968 | 22 | 11/1 | 6/3 | 1/0 |
At1g32640 | 1015 | 1015 | 0 | 49.8 | 2000 | 42 | 8/2 | 22/7 | 3/0 |
At2g22475 | 839 | 439 | 400 | 44.3 | 3416 | 33 | 7/2 | 16/7 | 1/0 |
At2g22540 | 1128 | 278 | 850 | 35.5 | 3216 | 25 | 18/2 | 4/0 | 1/0 |
At2g26300 | 1152 | 547 | 605 | 37.7 | 3456 | 32 | 19/3 | 6/2 | 2/0 |
At2g27100 | 1272 | 1049 | 223 | 43.8 | 3360 | 25 | 6/2 | 14/3 | 0/0 |
At3g06120 | 1143 | 513 | 630 | 35.0 | 3432 | 29 | 11/3 | 10/4 | 1/0 |
At3g13040 | 1093 | 581 | 512 | 39.8 | 2728 | 28 | 17/3 | 4/2 | 2/0 |
At3g24740 | 1195 | 1068 | 127 | 46.4 | 3472 | 46 | 10/3 | 23/8 | 2/0 |
At5g07280 | 1228 | 1228 | 0 | 46.0 | 2728 | 36 | 6/2 | 17/10 | 1/0 |
At5g11270 | 1075 | 667 | 408 | 39.8 | 1896 | 21 | 7/5 | 4/4 | 1/0 |
At5g23280 | 1077 | 762 | 315 | 42.5 | 3352 | 39 | 7/1 | 23/7 | 1/0 |
At5g41560 | 1299 | 262 | 1037 | 36.8 | 3200 | 32 | 25/5 | 0/1 | 1/0 |
At5g50570 | 1180 | 939 | 241 | 40.3 | 3384 | 40 | 12/1 | 23/1 | 2/1 |
Total | 15673 | 9764 | 5909 | 41.0 | 41608 | 450 | 164/35 | 172/59 | 19/1 |
Average | 1120 | 697 | 422 | 41.0 | 2972 | 32 | 12/3 | 12/4 | 1/0 |
Observed frequency (%) | 47.2 | 48.5 | 4.3 | ||||||
Expected frequency (%) | 48.8 | 46.1 | 5.1 | ||||||