Cytosolic N-terminal arginine-based signals together with a luminal signal target a type II membrane protein to the plant ER

Table 3 Oligonucleotides used to generate GFP fusions.

Primer	5'-3' sequence
	At glucosidase I as template
RGCS150	GACTAGTACACAAATGCCGCATAAC
RGCS90	GACTAGTAAAAGGAGTGATAACCCT
FΔ13GCS90/150	CGGGGTACCCCATGAAATCATCATCATTATCTCCC
FR/L4	CGGGGTACCCCATGACCGGAGCTAGCCTTCTGAGCGCGCTTGGTCTAATCAAATCATCA
FR/A4	CGGGGTACCCCATGACCGGAGCTAGCGCTGCGAGCGCGGCTGGTGCAATCAAATCATCA
FR/L6-7	CGGGGTACCCCATGACCGGAGCTAGCCTTCTGAGCGCGCGT
FR/L10L12	CGGGGTACCCCATGACCGGAGCTAGCCGTCGGAGCGCGCTTGGTCTAATCAAATCATCA
FR/L6L10	CGGGGTACCCCATGACCGGAGCTAGCCTTCGGAGCGCGCTTGGTCGAATCAAATCATCA
FR/L6L12	CGGGGTACCCCATGACCGGAGCTAGCCTTCGGAGCGCGCGTGGTCTAATCAAATCATCA
FR/L7L10	CGGGGTACCCCATGACCGGAGCTAGCCGTCTGAGCGCGCTTGGTCGAATCAAATCATCA
FR/L7L12	CGGGGTACCCCATGACCGGAGCTAGCCGTCTGAGCGCGCGTGGTCTAATCAAATCATCA
Fhs10GCS90	CGGGGTACCCCATGGCTCGGGGCGAGCGGCGGCGCCGCGCAAA
FGCS(70-150)	GGGGTACCCGGCTAGTTCGTCACGGG
FGCS(91-150)	GGGGTACCCCTGCTCCGAAAGTCATG
	β1,2xylosyltransferase as template
FGCS13XYLT35	CGGGGTACCCCATGACCGGAGCTAGCCGTCGGAGCGCGCGTGGTCGAATCAGTAAACGGAATCCGAAG
FCNX11XYLT35	CGGGGTACCCCATGAATGATCGTAGACCGCAAAGGAAACGCCCAAGTAAACGGAATCCGAAG-3'
RXYLT35	GGACTAGTTGAAAACGACGATGAGTG
FXYLT35'	GCTCTAGAGCATGAGTAAACGGAATCCG
RXYLT35'	GGGGTACCTGAAAACGACGATGAGTG

The BamHI, SpeI or KpnI restriction sites used for vector construction are underlined. Triplet codons for leucine or alanine are given in bold.

ISSN: 1471-2229