Figure 7
Punctate structures diappear when COPI-mediated retrograde transport is inhibited with BFA. CLSM analysis of Nicotiana tabacum leaf epidermal cells co-expressing GFP-fusions and mRFP-HDEL. Control cells co-expressing R/LGCS90 and mRFP-HDEL show green Golgi stacks and a red ER (-BFA, panels A-C). When cells are treated with BFA for 2 h, Golgi membranes are reabsorbed in the ER and the ER appears in yellow (+BFA, panels D-F). When cells co-expressing R/L6-7GCS90 (G-L) or R/L10-12GCS90 (M-R) and mRFP-HDEL are treated with BFA for 2 h, punctate structures disappear (J-L and P-R respectively).