Figure 1

Schematic representation of the fusion proteins analyzed in this study. AtGCSI: full-length A. thaliana α-glucosidase I fused to GFP. Δ13GCSI: GCSI minus the first 13 N-terminal aa (MTGASRRSARGRI-). GCS150: the first 150 aa of GCSI fused to GFP. GCS90: the first 90 aa of GCSI fused to GFP or mRFP. Δ13GCS150: GCS150 minus the first 13 N-terminal aa. Δ13GCS90: GCS90 minus the first 13 N-terminal aa. Hs10-Δ13GCS90: the first 10 N-terminal aa of Homo sapiens GCSI (MARGERRRRA-) fused at the N-terminus of Δ13GCS90. XYLT35: the first 35 aa of A. thaliana β-1,2-xylosyltransferase fused to GFP or mRFP [47]. XYLT35-GCSlum60: the first 35 aa of XYLT fused to the first 60 aa of the luminal domain of GCSI (Pro91 to Cys150) and to GFP. XYLT35-GCSlum81: the first 35 aa of XYLT fused to the first 81 aa of luminal domain of GCSI (Arg70 to Cys150) and to GFP. GCS13-XYLT35: the 13 first N-terminal aa of GCSI fused to XYLT35. ST-mRFP: the first 52 aa of a rat α-2,6-sialyltransferase (ST) fused to mRFP [90]. mRFP-HDEL: mRFP under the control of the sporamine signal peptide and the HDEL ER retention sequence. CT: cytosolic tail; TMD: transmembrane domain; CD: C-terminal domain.