Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®

Table 3 Comparison of results from independent HRM and Mutation Surveyor^® analysisof 140 TILLING lines.

Fragment	HRM Scanning			Mutation Surveyor^®			HRM Sensitivity⁴
	Mut¹	T²	F³	Mut¹	HRM detected	HRM un-detected	% sensitivity⁴	% false positive rate⁴
ABD6-1	15	7	8	8	7	1	87.5	5.7
ABD12-22	10	6	4	6	6	0	100	2.8
ABD2-9	15	5	10	6	5	1	83.3	7.1

¹Number of mutations identified by HRM or Mutation Surveyor^®.
²T = True mutants that sequences contain mutations confirmed by Mutation Surveyor^®.
³F = False mutants that sequences do not contain mutations confirmed by Mutation Surveyor^®.
⁴%sensitivity = true positive/(true positive + false negative); %false positive rate = false positive/total number of sample analysed; assuming Mutation Surveyor^® analyses are 100% correct.

ISSN: 1471-2229