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Table 3 Comparison of results from independent HRM and Mutation Surveyor® analysisof 140 TILLING lines.

From: Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®

Fragment HRM Scanning Mutation Surveyor® HRM Sensitivity4
  Mut1 T2 F3 Mut1 HRM detected HRM un-detected % sensitivity4 % false positive rate4
ABD6-1 15 7 8 8 7 1 87.5 5.7
ABD12-22 10 6 4 6 6 0 100 2.8
ABD2-9 15 5 10 6 5 1 83.3 7.1
  1. 1Number of mutations identified by HRM or Mutation Surveyor®.
  2. 2T = True mutants that sequences contain mutations confirmed by Mutation Surveyor®.
  3. 3F = False mutants that sequences do not contain mutations confirmed by Mutation Surveyor®.
  4. 4%sensitivity = true positive/(true positive + false negative); %false positive rate = false positive/total number of sample analysed; assuming Mutation Surveyor® analyses are 100% correct.