Table 2 17 mutations are identified in 192 TILLING lines in ABD6-9 after Mutation Surveyor® analysis of sequence traces and confirmation by HRM analysis.
No | Sample | Mutation Surveyor report | Position in ABD6-9 | Position in Gene (SSII-A) | Codon change | Amino acid change | Mutation type |
|---|---|---|---|---|---|---|---|
1 | 3D7 | (16)C>CT$7* | C34T | C5999T | cac/tac | H501Y | missense |
2 | 1D3 | (25)C>CT$10 | C43T | C6008T | ctg/ttg | L504L | silent |
3 | 3F10 | (65)C>CT$7 | C83T | C6048T | gcc/gtc | A517V | missense |
4 | 1C8 | (85)G>AG$7 | G103A | G6068A | gac/aac | D524N | missense |
5 | 1F10 | (115)C>CT$7 | C133T | C6098T | ctg/ttg | L534L | silent |
6 | 1D8 | (129)G>AG$7 | G147A | G6112A | aag/aaa | K538K | silent |
7 | 3E5 | (129)G>AG$7 | G147A | G6112A | aag/aaa | K538K | silent |
8 | 3A8 | (148)G>AG$7 | G166A | G6131A | ggg/agg | G545R | missense |
9 | 1D9 | (151)C>CT$28 | C169T | C6134T | ctt/ttt | L546F | missense |
10 | 3A4 | (159)C>CT$11 | C177T | C6142T | gac/gat | D548D | silent |
11 | 3H9 | (187)C>CT$7 | C205T | C6170T | cgc/tgc | R558C | missense |
12 | 1D5 | (300)G>AG$7 | G318A | G6283A | cgg/cga | R595R | silent |
13 | 3D8 | (306)C>CT$7 | C324T | C6289T | tgc/tgt | C597C | silent |
14 | 3F6 | (342)C>CT$7 | C360T | C6325T | gtc/gtt | V609V | silent |
15 | 1B2 | (361)C>CT$13 | C379T | C6344T | ctc/ttc | L616F | missense |
16 | 3B7 | (365)G>AG$7 | G383A | G6348A | ggc/gac | G617D | missense |
17 | 1E4 | (387)G>AG$7 | G405A | G6370A | ggg/gga | G624G | silent |