Figure 6

Progeny tests of mutants 4A7 and 4D7. Progeny tests of mutants 4A7 (C454T, Q641*) and 4D7 (G165A, W544*). (A) Twelve segregating M2 seedlings of 4A7 were analysed by HRM in ampilcon ABD3-9, four samples showed mutant-like melting peaks (thick lines). The thick black line is the known mutant control. (B) Ten segregating M2 seedlings of 4D7 were analysed by HRM in amplicon ABD6-1, seven samples showed mutant-like melting peaks (thick lines). The thick black line is the known mutant control. Representative sequence traces are shown on the right; homozygote is at the top, heterozygote in the middle and non-mutant at the bottom. Vertical arrows show the mutation positions.