Salt regulation of CNGC19. (A-E) GUS staining of 18-day old plants carrying the CNGC19p:GUS construct, which were grown in the absence (A, D) or presence (B, E) of 150 mM NaCl for 7 days. (C, F) Luciferase activity in CNGC19p:LUC transformed plants. Promoter activity was determined as relative luciferase luminescence units (RLU) in shoots (C) and roots (F) normalized to the respective protein content. Roots of 12-day old plants were either untreated (black bars), or treated with 200 mM NaCl (blue bars) or 300 mM mannitol (white bars). Luciferase activities were determined after stress application at time points indicated. Data represent mean ± SEM (n = 3).