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Table 3 List of primers utilized in the isolation of the 3' part of the genes isolated in this study

From: Dominance induction of fruitlet shedding in Malus × domestica (L. Borkh): molecular changes associated with polar auxin transport

Clone Primers Tm
LAX1 5'-TTTCTCTCTCCTCCCCAAGAAC-3' 68
LAX2/3 5'-CTTTGGATCTGTCATCCAACTTATAG-3' 71
PIN1/10 5'-GGCCTCACTTGGTCTCTAGTCTCA-3' 70
PIN4/7 5'-GTATTCGAGCCTCATTGGTCTCATC-3' 70
ARR3/4 5'-CGAGGGATGTCGGAGTGGAAGAG-3' 69
ARR6 5'-CAGCGGGACGAGAGCTCTGGAG-3' 69
  1. Specific primers used in the 3' RACE for the isolation of the 3' part of LAX, PIN, and ARR type A. The annealing temperature is also reported.