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Table 2 List of degenerate primers utilized in the isolation of genes

From: Dominance induction of fruitlet shedding in Malus × domestica (L. Borkh): molecular changes associated with polar auxin transport

Clone   Primers   L Tm
   forward reverse bp °C
LAX S 5'-AACCAYGTYATHCAGTGGTT-3' 5'-GGRATGAYRTARACDGTGAA-3' 840 58
  M NHVIQWF SFTVYIIP   
PIN S 5'-CARWGYATHTGGTAYAC-3' 5'-GCAYYARCATYCCAAADAT-3' 1400 57
  M QCIIWY VIFGMLM   
ARR type S 5'-CATGTTYTNGCBGTTGATG-3' 5'-CATNCCRGGCATDSAGTARTC-3' 200 60
A M HVLAVDDS TDYSMPGM   
  1. Degenerate primers used for the isolation of partial clones of LAX, PIN, and ARR type A identified in this study. S indicates the oligonucleotide sequence while M the conserved protein sequence chosen to design the primers. The amplification length of the product (L) expressed in bp and the annealing temperature are also reported.