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Table 2 List of degenerate primers utilized in the isolation of genes

From: Dominance induction of fruitlet shedding in Malus × domestica (L. Borkh): molecular changes associated with polar auxin transport

Clone

 

Primers

 

L

Tm

  

forward

reverse

bp

°C

LAX

S

5'-AACCAYGTYATHCAGTGGTT-3'

5'-GGRATGAYRTARACDGTGAA-3'

840

58

 

M

NHVIQWF

SFTVYIIP

  

PIN

S

5'-CARWGYATHTGGTAYAC-3'

5'-GCAYYARCATYCCAAADAT-3'

1400

57

 

M

QCIIWY

VIFGMLM

  

ARR type

S

5'-CATGTTYTNGCBGTTGATG-3'

5'-CATNCCRGGCATDSAGTARTC-3'

200

60

A

M

HVLAVDDS

TDYSMPGM

  
  1. Degenerate primers used for the isolation of partial clones of LAX, PIN, and ARR type A identified in this study. S indicates the oligonucleotide sequence while M the conserved protein sequence chosen to design the primers. The amplification length of the product (L) expressed in bp and the annealing temperature are also reported.