Figure 3

Electron microscopic observation showed obvious structural changes of Golgi apparatus in root cap peripheral cells of the kinesin-13a-1 mutant line. (A) The peripheral cells of the kinesin-13a-1 mutant lines contained a few large vacuoles and few vesicles. (B) The peripheral cells of the wild type root cap contained numerous vesicles. (C), (E) Golgi-associated vesicles were rare and small in the peripheral cells of the kinesin-13a-1 mutant. (D), (F) The wild type root-cap peripheral cells contained abundant and bulky secretory vesicles around the Golgi stack. G: Golgi apparatus. P: peripheral cells. SV: secretory vesicles. Bars: 1 μm (A, B); 200 nm (C, D); 150 nm (E, F).