Table 3 Information for six markers that were analyzed in the (Valmaine × Salinas 88) × Salinas mapping population with the HRM approach.
From: Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1
Marker | EST/Contig in CGPDB | Primer (5' - 3') | Ta (°C) | Mg (mM) | Amplicon size (bp) |
|---|---|---|---|---|---|
LK1457 | QG_CA_Contig4638 | F - AGGAGCAAAGGAAAGGCTTC | 64 | 3 | 636-648 |
R - TGCAACTTCTTCAGCCAATG | |||||
Cntg4252 | CLS_S3_Contig4252 | F - AGAACCAGGTCGAATCATGG | 61 | 1.5 | 208 |
R - TTCTCGCCGTTGAGAAGAAT | |||||
Probe - AAGTGGCTATACAGCTTTGATCATAACGA | |||||
Cntg10192 | CLS_S3_Contig10192 | F - CTCGTTTTCAACACCGACAA | 61 | 1.5 | 185 |
R - TAGGTGGGTCCGACTTTGAG | |||||
CLSM9959 | CLSM9959.b1_N18.ab1 | F - TGCTCAATTACACTCGAACCA | 61 | 1.5 | 326 |
R - CTTCATGGAGAGAAATACAAGGTC | |||||
CLSZ1525 | CLSZ1525.b1_J22.ab1 | F - GAAGAAACTCATGAATCTGCTCAA | 62 | 3 | 157-158 |
R - TTTGCTCAAGAACTCTTAAACCATT | |||||
Cntg11275 | CLS_S3_Contig11275 | F - CCAAACCATAGGGACGAAAA | 61 | 1.5 | 252-260 |
R - GGAGGTATGTTGAGGTACATGAC |