Overlapping TIP isoforms are mainly detected at the tonoplast of the central vacuole. Transgenic seedlings co-expressing the indicated TIP-YFP and TIP-RFP constructs were grown for 8 days on MS medium--agar plates. Roots were excised and visualised by CLSM. (A, D, G, J): YFP fluorescence (green); (B, E, H, K): RFP fluorescence (red); (C, F, I, L): merged images. Arrowheads in panel I indicate structures labelled by TIP2;3-RFP but not TIP2;2-YFP. Scale bars:10 μm.