Figure 10

A) Separation of pigmented photosynthetic complexes of thylakoids prepared from leaves of WT and pdx1 by solubilization in 0.8% dodecylmaltoside and ultracentrifugation on sucrose gradient. Thylakoids were prepared from leaves of WT and pdx1 grown in low light (c, 200 μmol photons m^-2 s^-1) or acclimated for 7 d to high light (hl, 1000 μmol m^-2 s^-1). B1, free pigments; B2, monomeric Lhcb antennae; B3, LHCII trimers; B5, PSII core (monomeric), B6, PSI-LHCI supercomplex. The B4 band (LHCII-CP29-CP24 supercomplex, see [85]) is not visible in this gradient. B) Ultracentrifugation gradient of thylakoids (pdx1, c and hl) solubilized in 1.2% dodecylmaltoside. In the control pdx1 sample, an additional band appeared in the bottom of the gradient, which was hardly visible at 0.8% dodecylmaltoside and which corresponded to dimeric PSI-LHCI. This is presumably due to an artificial aggregation the high detergent concentration used in this preparation as previously found [86]; the same phenomenon was observed with WT thylakoids (data not shown). C and D) SDS-PAGE separation of the B2, B3 and B6 bands using two different buffer systems: tricine (C) and urea (D). See ref. [87] for identification of the bands. BBY = PSII-enriched membranes used as a reference for the PSII proteins.