Figure 2
Callose and pectins during microsporogenesis in Annona cherimola. (A-D) Anticallose in dyad/tetrad phases. (A) A furrow of callose developed inwards, forming a wall between the dyad cells. (B) Dyad phase, showing (B) an incipient, and (C) a well developed callose wall. (D) Tetrad microspore showing in the section plane three of the microspores separated by callose walls. (E) PMC and other anther tissue walls showing methyl-esterified pectins. (F) PMC wall also shows unesterified pectins. Specific cell components were localized using antibodies against callose (A, B, C, D), methyl-esterified pectin (JIM7) (E), and unesterified pectin (JIM5) (F). A-D Bar = 10 Ī¼m. E-F: Bar = 20 Ī¼m.