Table 5 The list of PCR primers used in real-time RT-PCR.
From: Identification of flowering genes in strawberry, a perennial SD plant
Gene | Forward primer | Reverse primer |
|---|---|---|
UBI | CAGACCAGCAGAGGCTTATCTT | TTCTGGATATTGTAGTCTGCTAGGG |
LFY | CGGCATTACGTTCACTGCTA | CCTGTAACACGCCTGCATC |
SOC1 | CAGGTGAGGCGGATAGAGAA | AGAGCTTTCCTCTGGGAGAGA |
AP1 | CGCTCCAGAAGAAGGATAAGG | CATGTGACTGAGCCTGTGCT |
AP1 | TCTGAAGCACGTAAGGTCTA | ATCCTGATCATAACCTCCAG |
LHY | AAAGCTGGAGAAGGAGGCAGTC | CCGAGGATAAGGATTGCTTGGT |
ZTL | TGCATGGGGTAGTGAAACAA | CACCTCCGACAGTGACCTTT |
FKF1 | ACCCACATCGTTTGTGGTCT | ACATCAGGATCCACCAGAGG |
ELF3 | TCCTCCAAGGAACAAGATGG | CCATTCCCCTGATTTGAGAG |
ELF6 | TTCGAAGGTCTTGGCAATGG | GCGCCTGAGTTTTATCCAACAC |
COL4 | GACCGAGAAATCCACTCTGC | CTCTCCGTCCGACAAGTAGC |
CO | GACATCCACTCCGCCAAC | GTGGACCCCACCACTATCTG |
PFT1 | GCGACATGCCAAGGTTAGAATT | TCAGCGCCTCACACTCTTACAC |
HRB1 | GAATGGTGGACATCAGCAATCC | CCTCCGAAAGAATTGCTCAACA |
FYPP3 | ACAAAATGGCCCCTCATGTG | TGTGCTATGTGTCCATGGTGGT |
FRL | CGCTAGTCAAGGTCGAGGAG | CGACTTCATCTCCATCAGCA |
ELF8 | GCTCAGAATGCTCCTCCTGT | TGAGTATTGCAGCCACTTGC |
VRN5 | AGCCCTTGATGTCATCAGCTG | CCGATGAATGGTTGGCTAATG |
MSI1 | TCTCCACACCTTTGATTGCCA | ACACCATCAGTCTCCTGCCAAG |
LHP1 | GGAGAGCCAGAACCAGGAG | CTCACCTTCTTCCCCTTCCT |
FVE | GATCCAGCAGCAACCAAGTCTC | CCTCTTGGTGCAACAGAAGGAC |
SVP | CGTGCTAAGGCAGATGAATGG | TGAAGCACACGGTCAAGACTTC |
SPY | TGCGGTGTCAAATTGCATCA | GGCAACACTCAAGATGGATTGC |
GA3ox | CCTCACAATCATCCACCAATCC | CGCCGATGTTGATCACCAA |
GA2ox | CACCATGCCCAGAGCTTCA | AGGCCAGAGGTGTTGTTGGAT |
TFL1 | TGCAGAAACAAACGAGTTCGG | CCAAGAGCATCGATCATTTGGT |
AP2 | CCCGAAATCCTTGATTGTTCC | AACACTGCAATCGAACAACAGC |