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Table 3 Kinetic parameters of purified recombinant RsHTMT.

From: Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus(daikon radish)

Substrate

Km (mM)

Vmax (pmol/min/mg)

Vmax/Km

SAM

0.19

--

--

Cl-

1657.40

3,381

2.04

Br-

177.34

34,965

1.97 × 102

I-

4.47

139,286

3.12 × 104

[SH]- (NaSH)

12.24

158,732

1.30 × 104

[SCN]-

0.04

185,185

4.41 × 106

  1. Kinetic parameters for SAM were measured at a constant iodide concentration (20 mM). Parameters for each of the methyl acceptors were measured at constant SAM concentration (500 μM).
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BMC Plant Biology

ISSN: 1471-2229

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