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Table 2 PSSM and SIFT scores of WKS mutations.

From: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat

Gene Domain Line Nucleotide Change Amino Acid Change PSSM SIFT Reaction to PST
WKS1 Kinase T6-569 G 163 A V 55 I 11.5 0.00 Susceptible
   T6-89 G 508 A D 170 N 10.4 0.46 Resistant
   T6-312 G 585 A G 199 R 19.7 0.00 Susceptible
   T6-480-1 C 632 T T 211 I 12.6 0.01 Susceptible
   T6-138 G 914 A R 305 H 13.6 0.01 Susceptible
  START T6-567 G 4437 A D 477 N 12.3 0.00 Susceptible
WKS2 Kinase T6-960 C 13 T R 5 * ---a --- Resistant
   T6-480-2 G 72 A W 24 * --- --- Resistant
  START T6-826 G 2221 A W 379 * --- --- Resistant
  1. a PSSM and SIFT scores are not reported for mutations that cause premature stop codons
  2. Six WKS1 and three WKS2 mutants were scored as susceptible or resistant based on their reaction to Puccinia striiformis f. sp. tritici (PST) in Fu et al. [32]. In the nucleotide/amino acid change columns, the first letter indicates the wild type nucleotide/amino acid, the number its position from the start codon/methionine, and the last letter the mutant nucleotide/amino acid. High PSSM (>10) and low SIFT scores (<0.05) predict mutations with severe effects on protein function.