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Table 1 Characteristics of TILLING targets and mutation frequencies in the hexaploid and tetraploid TILLING populations

From: A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat

Gene Pop. Chr. Size (bp) GC content (%) M2plants screened Mutations Mutation Frequency
WKS1 6B 1371 39.8 1536 28 1/60 kb
  6B 1270 40.7 1536 43 1/37 kba
WKS2 6B 1460 39.1 768 25 1/36 kb
  6B 1532 39.8 768 25 1/42 kbb
SBEIIa 2A 1593 37.7 1536 40 1/49 kb
  2B 1638 37.7 768 17 1/59 kb
  2D 1614 37.2 768 8 1/124 kb
SBEIIa 2A 1637 37.9 1368 31 1/58 kb
  2B 1641 37.8 1368 27 1/67 kb
SBEIIb 2A 1909 36.8 600 15 1/61 kb
  2B 1972 36.8 1152 20 1/91 kb
  1. a 384 M2 plants were screened with LI-COR and 1152 M2 plants were screened using the polyacrylamide/ethidium bromide method.
  2. b All 768 M2 plants were screened with LI-COR.