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Table 3 List of the spots identified in the leaves and their change in abundance after the exposure to 10 mM nitrate for 30 h.

From: Evaluation of protein pattern changes in roots and leaves of Zea maysplants in response to nitrate availability by two-dimensional gel electrophoresis analysis

Spot ID Accession number Protein description Abbr.a M r b/pIb M r c/pIc Change in level [Relative volume (%)]
       Control 10 mM NO3
Nitrogen and amino acid metabolism
1094 BAB11740 TaWIN2 TaWIN2 29.9/4.7 28.7/4.8 0.182 ± 0.009 0.090 ± 0.014
254 AAL73979 Methionine synthase protein MetS 83.4/5.9 83.8/5.9 0.148 ± 0.020 0.073 ± 0.008
C-compound and carbohydrate metabolism
650 AAC27703 Putative cytosolic 6-phosphogluconate dehydrogenase 6PGD 47.4/6.0 52.9/6.2 0.088 ± 0.005 0.043 ± 0.007
Photosynthesis
134 P04711 Phosphoenolpyruvate carboxylase 1 PEPCase-a 104.4/5.8 109.3/5.8 0.990 ± 0.083 2.770 ± 0.295
138 P04711 Phosphoenolpyruvate carboxylase 1 PEPCase-b 104.4/5.7 109.3/5.8 2.220 ± 0.278 1.090 ± 0.205
500 P05022 ATP synthase subunit alpha, chloroplastic ATPsyn α 55.9/6.1 55.7/5.9 0.042 ± 0.007 0.015 ± 0.003
1065 NP_001063777 Putative triosephosphate isomerase, chloroplast precursor d TIM 31.0/4.9 32.4/7.0 0.028 ± 0.009 0.088 ± 0.014
1244 Q00434 Oxygen-evolving enhancer protein 2, chloroplast precursor OEE2 26.6/6.5 27.3/8.8 0.201 ± 0.013 0.090 ± 0.011
1612 BAA08564 23 kDa polypeptide of photosystem II 23pPSII 26.3/6.5 27.0/9.5 0.147 ± 0.008 0.055 ± 0.006
Protein folding and stabilization
462 NP_001056601 RuBisCO subunit binding-protein beta subunit d CPN-60 β 58.5/5.1 64.1/5.6 0.079 ± 0.014 0.164 ± 0.015
467 AAP44754 Putative rubisco subunit binding-protein alpha subunit precursor CPN-60 α 58.2/4.8 61.4/5.4 0.046 ± 0.004 0.096 ± 0.004
Metabolism of vitamins, cofactors, and prosthetic groups
999 Q41738 Thiazole biosynthetic enzyme 1-1, chloroplast precursor TH1-1 33.0/5.1 32.8/4.9 e 0.010 ± 0.001 0.048 ± 0.003
Secondary metabolism
313 AAL40137 Phenylalanine ammonia-lyase PAL 70.2/6.0 74.9/6.5 0.076 ± 0.008 0.023 ± 0.002
Lipid metabolism
219 ABC59693 Lipoxygenase LOX 94.6/5.8 102.1/6.1 0.023 ± 0.011 0.149 ± 0.011
  1. Statistical information about LC-ESI-MS/MS analysis are reported in Additional files 2 and 3. Changes in the relative spot volumes are the mean ± SE of six 2-DE gels derived from three independent biological samples analyzed in duplicate (n = 6). Proteins were classified according to MIPS funcat categories.
  2. a: Protein abbreviation
  3. b: Experimental molecular weight (kDa) or isoelectric point
  4. c: Theoretical molecular weight (kDa) or isoelectric point
  5. d: Information obtained by alignment of the sequence through BLAST analysis against NCBI nr database
  6. e: Values referred to the mature form of the protein