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Table 2 List of the spots identified in the roots and their change in abundance after the exposure to 10 mM nitrate for 30 h.

From: Evaluation of protein pattern changes in roots and leaves of Zea maysplants in response to nitrate availability by two-dimensional gel electrophoresis analysis

Spot ID

Accession number

Protein description

Abbr.a

M r b/pIb

M r c/pIc

Change in level

[Relative volume (%)]

      

Control

10 mM NO3

Glycolysis, gluconeogenesis, C-compound and carbohydrate metabolism

53

BAA28170

Phosphoenolpyruvate carboxylase

PEPCase-UB

115.4/5.7

109.4/5.7

0.223 ± 0.022

0.084 ± 0.032

 

P69319

Ubiquitin

  

8.5/6.6

  

216

P30792

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

PGAM-I

63.0/5.1

60.6/5.3

0.124 ± 0.086

0.245 ± 0.011

231

AAL99745

Pyruvate decarboxylase

PDC

62.4/5.5

65.0/5.7

0.080 ± 0.043

0.167 ± 0.024

392

EAZ18378

6-phosphogluconate dehydrogenased

6PGD

50.1/6.1

50.1/5.5

0.080 ± 0.031

0.275 ± 0.033

1162

NP_196815

Glucose-6-phosphate 1-dehydrogenase

G6PD

60.3/6.7

67.2/8.5

0.002 ± 0.001

0.010 ± 0.014

Nitrogen metabolism, amino acid metabolism and protein/peptide degradation

268

ACG29734

Ferredoxin-nitrite reductase

NiR

59.7/6.7

66.2/6.5

0.035 ± 0.054

0.124 ± 0.084

483

P25462

Glutamine synthetase, chloroplastic

GS2

42.2/5.2

41.0/5.4 e

0.066 ± 0.015

0.137 ± 0.059

538

P38559

Glutamine synthetase root isozyme 1

GS1-1

38.7/5.1

39.2/5.6

0.210 ± 0.010

0.480 ± 0.039

707

BAA06876

Aspartic protease

AP

31.6/4.6

54.1/5.1

0.051 ± 0.043

0.015 ± 0.065

Secondary metabolism

171

AAL40137

Phenylalanine ammonia-lyase

PAL-a

68.6/5.9

74.9/6.5

0.476 ± 0.034

0.184 ± 0.012

172

AAL40137

Phenylalanine ammonia-lyase

PAL-b

68.6/5.8

74.9/6.5

0.904 ± 0.136

0.277 ± 0.026

1160

AAL40137

Phenylalanine ammonia-lyase

PAL-c

68.0/5.8

74.9/6.5

0.713 ± 0.103

0.275 ± 0.034

Cell rescue, defense and virulence

390

NP_001061002

Putative monodehydroascorbate reductase d

MDHAR

50.1/6.2

52.8/6.8

0.127 ± 0.016

0.275 ± 0.033

960

AAZ98790

hemoglobin 2

Hb2

24.8/4.9

20.6/5.0

0.018 ± 0.061

0.099 ± 0.068

Unknown

774

Q01526

14-3-3-like protein GF14-12

GF14-12

29.6/4.6

29.6/4.7

0.345 ± 0.034

0.146 ± 0.028

  1. Statistical information about LC-ESI-MS/MS analysis are reported in Additional files 2 and 3. Changes in the relative spot volumes are the mean ± SE of six 2-DE gels derived from three independent biological samples analyzed in duplicate (n = 6). Proteins were classified according to MIPS funcat categories.
  2. a: Protein abbreviation
  3. b: Experimental molecular weight (kDa) or isoelectric point
  4. c: Theoretical molecular weight (kDa) or isoelectric point
  5. d: Information obtained by alignment of the sequence through BLAST analysis against NCBI nr database
  6. e: Values referred to the mature form of the protein
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BMC Plant Biology

ISSN: 1471-2229

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