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Table 2 List of the spots identified in the roots and their change in abundance after the exposure to 10 mM nitrate for 30 h.

From: Evaluation of protein pattern changes in roots and leaves of Zea maysplants in response to nitrate availability by two-dimensional gel electrophoresis analysis

Spot ID Accession number Protein description Abbr.a M r b/pIb M r c/pIc Change in level [Relative volume (%)]
       Control 10 mM NO3
Glycolysis, gluconeogenesis, C-compound and carbohydrate metabolism
53 BAA28170 Phosphoenolpyruvate carboxylase PEPCase-UB 115.4/5.7 109.4/5.7 0.223 ± 0.022 0.084 ± 0.032
  P69319 Ubiquitin    8.5/6.6   
216 P30792 2,3-bisphosphoglycerate-independent phosphoglycerate mutase PGAM-I 63.0/5.1 60.6/5.3 0.124 ± 0.086 0.245 ± 0.011
231 AAL99745 Pyruvate decarboxylase PDC 62.4/5.5 65.0/5.7 0.080 ± 0.043 0.167 ± 0.024
392 EAZ18378 6-phosphogluconate dehydrogenased 6PGD 50.1/6.1 50.1/5.5 0.080 ± 0.031 0.275 ± 0.033
1162 NP_196815 Glucose-6-phosphate 1-dehydrogenase G6PD 60.3/6.7 67.2/8.5 0.002 ± 0.001 0.010 ± 0.014
Nitrogen metabolism, amino acid metabolism and protein/peptide degradation
268 ACG29734 Ferredoxin-nitrite reductase NiR 59.7/6.7 66.2/6.5 0.035 ± 0.054 0.124 ± 0.084
483 P25462 Glutamine synthetase, chloroplastic GS2 42.2/5.2 41.0/5.4 e 0.066 ± 0.015 0.137 ± 0.059
538 P38559 Glutamine synthetase root isozyme 1 GS1-1 38.7/5.1 39.2/5.6 0.210 ± 0.010 0.480 ± 0.039
707 BAA06876 Aspartic protease AP 31.6/4.6 54.1/5.1 0.051 ± 0.043 0.015 ± 0.065
Secondary metabolism
171 AAL40137 Phenylalanine ammonia-lyase PAL-a 68.6/5.9 74.9/6.5 0.476 ± 0.034 0.184 ± 0.012
172 AAL40137 Phenylalanine ammonia-lyase PAL-b 68.6/5.8 74.9/6.5 0.904 ± 0.136 0.277 ± 0.026
1160 AAL40137 Phenylalanine ammonia-lyase PAL-c 68.0/5.8 74.9/6.5 0.713 ± 0.103 0.275 ± 0.034
Cell rescue, defense and virulence
390 NP_001061002 Putative monodehydroascorbate reductase d MDHAR 50.1/6.2 52.8/6.8 0.127 ± 0.016 0.275 ± 0.033
960 AAZ98790 hemoglobin 2 Hb2 24.8/4.9 20.6/5.0 0.018 ± 0.061 0.099 ± 0.068
Unknown
774 Q01526 14-3-3-like protein GF14-12 GF14-12 29.6/4.6 29.6/4.7 0.345 ± 0.034 0.146 ± 0.028
  1. Statistical information about LC-ESI-MS/MS analysis are reported in Additional files 2 and 3. Changes in the relative spot volumes are the mean ± SE of six 2-DE gels derived from three independent biological samples analyzed in duplicate (n = 6). Proteins were classified according to MIPS funcat categories.
  2. a: Protein abbreviation
  3. b: Experimental molecular weight (kDa) or isoelectric point
  4. c: Theoretical molecular weight (kDa) or isoelectric point
  5. d: Information obtained by alignment of the sequence through BLAST analysis against NCBI nr database
  6. e: Values referred to the mature form of the protein