Evaluation of protein pattern changes in roots and leaves of Zea maysplants in response to nitrate availability by two-dimensional gel electrophoresis analysis

Table 1 Evaluation of the procedure for the extraction of soluble proteins from roots and leaves of plants grown in the two conditions compared in the proteomic analysis.

Organ	Condition	FW/DW	Total proteins (mg g^-1FW)	Extraction yield of soluble proteins (%)
Root	C plants	8.63 ± 0.05	5.32 ± 0.34	13.39 ± 0.72
	N plants	8.43 ± 0.34	6.04 ± 0.41	14.63 ± 0.69
Leaf	C plants	8.45 ± 0.09	9.39 ± 0.45	19.17 ± 0.99
	N plants	8.64 ± 0.15	9.35 ± 0.43	20.96 ± 0.43

In the table, the fresh/dry weight (FW/DW), the content of total protein (mg g ^-1FW) and the % yield of the extraction of soluble proteins (% of extracted soluble proteins respect to the total content) for the roots and the leaves of the plants compared by proteomic analysis are reported. The fresh weight of the roots was 0.56 ± 0.03 and 0.60 ± 0.04 g in C and N plants, respectively. The fresh weight of the leaves was 0.79 ± 0.03 and 0.86 ± 0.04 g in C and N plants, respectively.
C plants: plants kept in the absence of nitrogen; N plants: plants grown for the last 30 h in the presence of 10 mM NO₃^-. Values are the mean ± SE of three independent biological samples analyzed in triplicate (n = 9).

ISSN: 1471-2229