Expression of genes associated with carbohydrate metabolism in cotton stems and roots

BMC Plant Biology

Table 4 Primer Sequences

Gene	contig	sense 5'-3'	antisense 5'-3'	efficiency
sucrose phosphate phosphatase	5239	TGAAAGGGTGCTATGGAGAC	ACACAACAACATCGCTCATC	1.94
phospho gluco mutase	4985	CCGTGGATGGAAGTGTGG	TGGTTCAAATTGCTCGATGTAG	2.01
phospho gluco mutase	598	CGAGGACGGATCACGATTG	AGAGCAACTTCCACAAGAGG	1.96
ADPglucose pyrophosphorylase	12082	TGGACTGGTGAAGATGGATGG	CCGAGGAGCGTGGTATCAG	2.04
trehalose phosphate synthase	16866	GAACATCTCCTGGTGACAATG	GCAAACACATCCGCTACTG	1.95
trehalose phosphate synthase	4991	CCAGCGGTTGTCCATAGAG	ACCAACACACATCACGAAATC	2.01
amylase	6516	CAGAGGATCATTAATTGGATTG	ATTACACCTGGAGGCTTC	1.96
starch phosphorylase	16	TCAAAAGTGGCGTATTCGGATC	TGGGAAGTCTTTGCCAACAAG	2.04
water dikinase	16302	GCTGAGTTCTGGAATGCCTTG	CCTGGTGCTGAAATATGCTCTC	2.14
ribosomal protein	2930	AGTGCTTCTCTGATTGCTCAAGAC	TCGACCTGAACAACATATACGGATC	1.92
expansin	16061	TTGCTTTGTATTGTTCTGTGGTGTG	ACTTGGGCTGCGGGCTAC	1.87

Primers used for qPCR. Gene is a description of the gene function, contig references expression in table 2, sense is the forward primer, antisense is the reverse primer and efficiency is the efficiency of the primer pair in qPCR.

ISSN: 1471-2229