Figure 2

QPCR of selected genes. QPCR confirmed the differential expression of seven transcripts putatively encoding an ADPGp, an α-amylase, a starch phosphorylase (st-phos), GWD, a phosphoglucomutases (PGM 4985) and two trehalose phosphate syntases (TSP 16866 and TSP 4991) in field-grown cotton roots. The SPP fell just short of the two fold cut-off for differential expression. One PGM (PGM598) identified as differentially expressed on microarrays was not differentially expressed in these root samples between -4 week and -2 week. QPCR also confirmed the relatively constant expression (less than two fold variation) predicted by microarray analysis of a ribosomal protein gene (RIB) and an expansin gene (EXP). The RNAs analyzed were from cotton root harvested at the indicated times.