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Table 1 The kinetic parameters Km and Vmax and the relation (Vmax/Km) of CsGT45, toward kaempferol and quercetin with a fixed UDPG concentration.

From: Cloning and characterization of a glucosyltransferase from Crocus sativusstigmas involved in flavonoid glucosylation

substrate Km (μM) Vmax (pkat/mg protein) Vmax/Km
Kaempferol 7-OH 15.6 ± 1.2 366 ± 19.8 23.46
Quercetin 4'-OH 86.95 ± 8.4 186 ± 12.4 2.14
Quercetin 3'-OH 30.3 ± 3.6 22.9 ± 2.6 0.75
Quercetin 7-OH 21.50 ± 2.3 104 ± 5.46 4.83
  1. Enzyme assays were carried out using purified CsGT45 (7 μg), substrate (20 to 100 μM) and UDP-glucose (2.5 mM). Reactions mixtures were incubated at 30°C, and performed in triplicate.