Figure 5
Expression analysis of CsGT45 in plant tissues. (A) The level of CsGT45 was analysed in the stigma tissue of C. sativus in different developmental stages: yellow (y), orange (o), red (r), two days before anthesis (-2da), anthesis (da), one day after anthesis (+1da), and three days after anthesis (+3da), and in closed and open stamen (stc and sto), corm, tepals (pt) and style. Equal amounts of total RNA were used in each reaction. The levels of the constitutively expressed RPS18 coding gene were assayed as controls. (B) HPLC-ESI-MS chromatograms of MeOH extract of C. cancellatus stigmas at anthesis. (C) HPLC-ESI-MS chromatograms of MeOH extract of C. niveus at anthesis. (D) HPLC-ESI-MS chromatograms of MeOH extract of C. speciosus stigmas at anthesis. (E) HPLC-ESI-MS chromatograms of MeOH extract of C. cartwrightianus stigmas at anthesis. The peaks 1, kaempferol 3-O-sophoroside-7-O-glucopyranoside; 2, kaempferol 3,7,4'-triglucoside; and 3, kaempferol 7-O-sophorosid. The compound 4-methylumbelliferyl β-D-glucuronide was used as internal standard (IS). (F) Transcript levels of CsGT45 in the stigma tissue of different Crocus species: 1, C.niveus; 2, C. cancellatus; 3, C. speciosus; 4, C. sativus and 5, C. cartwrightianus. To ensure the detection of the transcripts, 40 PCR cycles were carried out.