Table 2 Oligonucleotide primers used in experimental analyses.
Oligonucleotide | Sequence | Accession No. | Gene | Amplicon, bp |
|---|---|---|---|---|
PKVexonF | 5'-GAAATCCTAGCAGTGGATCGG-3' | EU196240 | pkv | 531 |
PKVexonR | 5'-CAGCACTTCTTACTAAAGCCC-3' | " | " | " |
Pr1a | 5'-CAAACTCCTCGAGAGAATTT-3' | X71592 | Pr1a1 | 299 |
PrR | 5'-ACCACTTGAGTATAATGTC-3' | " | " | " |
Pr1bF | 5'-CAAAATTCACCCCAAGACTA-3' | X12486 | Pr1b | 300 |
LAPF | 5'-ATGCAGAACATGTATGTGCAG-3' | SLU50151 | LAP | 397 |
LAPR | 5'-TTTGCTGCACCTAAAACAGC-3' | " | " | " |
ACTRQF | 5'-GTGGCGGTTCGACTATGTTT-3' | EU938079 | ACTIN1 | 186 |
ACTRQR | 5'-ATTCTGCCTTTGCAATCCAC-3' | " | " | " |
PKVRQF | 5'-TCCGTTGTTCTGTCAATCCA-3' | EU196240 | pkv | 208 |
PKVRQR | 5'-CCTCCCACAAAAAGACCAAA-3' | " | " | " |
GA20RQF | 5'-TTCCGGTTCCACTTATCGAC-3' | AB032198 | GA20 oxidase 1 | 153 |
GA20RQR | 5'-GGCGTTGGAGATGATATTA-3' | " | " | " |
GA3RQF | 5'-TCAAAGAAGGGAGTGGTTGG-3' | AB032198 | GA3β hydroxylase | 155 |
GA3RQR | 5'-GGCTACAGAAAGGCGATGTC-3' | " | " | " |
NTH15RQF | 5'-CCCTCAGGCTGAAGATCAAG-3' | AB004785 | NTH15 | 152 |
NTH15RQR | 5'-GTCTGGTCCACCAGTCCAGT-3' | " | " | " |
RSGRQF | 5'-TGCTGAGTTGGCTTTGATTG-3' | AB040471 | RSG | 242 |
RSGRQR | 5'-CTCCTTGTTCCAAAGCTTGC-3' | " | " | " |
EXPRQF | 5'-TGTTGGAGGTGCTGGTGATA-3' | AF096776 | LeExp2 | 215 |
EXPRQR | 5'-CCCCTCAAAAGTTTGTCCA-3' | " | " | " |