Induction of SAR in npr1-1 plants transformed with GmNPR1-1 and GmNPR1-2 genes. Leaf number 3 and 4 were inoculated with 40 μl 10 mM MgCl2 or an avirulent strain Pst DC3000 containing AvrRpt2 (107 cfu/ml). Three days after inoculation, two younger systemic leaves (leaf number 5 and 6) were inoculated with the virulent strain Pst DC3000 (0.5 × 105cfu/ml). Transformants that showed PR1-1 expression following INA treatment (e.g. transformant #6 containing GmNPR1-1 or transformant #4 containing GmNPR1-2 as shown in Figure 7A) also showed SAR activities. The study was conducted with four biological replications. Bars without a common letter on the top are statistically different (Fisher's LSD test, P = 0.05). Standard errors are represented by error bars.