Figure 7
Induction of the pathogenesis-related genes by INA or infection in Arabidopsis npr1-1 mutant carrying GmNPR1 genes. A), Induction of PR-1 gene by INA. RNA gel blot analysis was performed using the Arabidopsis PR-1 gene as the probe. GmNPR1-1; two independent transformants; GmNPR1-2, four independent transformants. Note that PR-1 is induced in GmNPR1-1 and GmNPR1-2 complemented npr1-1 plants. B), Induction of beta glucanase 2 (BGL2) following infection. The leaves of the Arabidopsis npr1-1 mutant carrying the BGL2-GUS fusion gene with the BGL2 promoter transformed with no GmNPR1 gene (a and d), GmNPR1-1 (b and e), or GmNPR1-2 (c and f) were inoculated with Pst just before bolting. a, b, and c were inoculated with a virulent Pst strain. d, e, and f, were inoculated with an avirulent Pst strain. The plants were infiltrated with either Pst DC3000 or Pst DC3000 carrying the AvrRpt2 gene (105 cfu/mL (OD600 = 0.002). Results were comparable in three independent experiments.